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Protein Purification

Protein Purification

Automated High Throughput Purification System for

Proteins and other Biomolecules

  • Antibody Purification

  • Antigen and Enzyme Extraction

  • Metabolite Profiling

  • Oligonucleotide Purification

Up to 40X faster than manual protein purification

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OttoPure Protein Purification System

OttoPure - Automated Protein Purification is engineered to process up to 8 samples simultaneously, accommodating volumes up to 1L and offering the versatility of 9 buffer solutions.

Compatible with a broad range of columns from 1mL to 70mL, including GE HiTrap™ and standard SPE cartridges, OttoPure employs positive-pressure to ensure uniform flow rates and synchronized processing, eliminating the need for supervision.

 

With options for automated column regeneration and customizable features, OttoPure provides a robust and versatile solution for automating protein purification.

What does our customer say about OttoPure System for automating protein purification?

The AlphaThera team shared data on their use of OttoPure system for protein purification

SPE-03 protein purification in AlphaThera

 Protein Purification system at AlphaThera Lab

DNA

"With the SPE-03 (now OttoPure), we can run purification overnight, so it saves us a day, improves consistency, and removes one of the more redundant, time-consuming tasks in our workflow."
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AlphaThera

Overall feedback from AlphaThera team

  • High production level with low batch-to-batch variability (See Figure 1)

  • Significant time savings, with total purification time being reduced from 2 work days to just overnight (See Figure 2)

  • Easy scale up, allowing a 5x scale up in production to be easily accommodated (See Figure 3)

  • Easy to maintain the instrument

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AlphaThera is a biotechnology company focused on simplifying and standardizing antibody labeling to enable confident bioconjugation reactions. To optimize their protein purification process and enhance efficiency, the company deployed PromoChrom's OttoPure automated protein purification system. They also returned to buy a second system just months of installation five times.

 

AlphaThera has seen tremendous improvements in their production levels, batch-to-batch consistency, and purification time with the use of OttoPure. They were also able to scale up production by five times while keeping instrument maintenance simple.

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Highly Inert and Corrosion Resistant

Compatible with biological samples, acids, bases and salts

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Operates under temperatures low as

                 4°C

For proteins such as enzymes and antigens that require low temperature preservation

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Easily Programmable Touch Interface

Saves up to 100 methods for automated protein purification, system cleaning and column regeneration

Automated Protein Purification Steps:

Choose from up to 9 buffers to condition columns

Elute

Elute columns using selected buffer(s). Can collect up to 2 separate fractions automatically.

Wash Column

Choose from up to 9 buffers to remove impurities

Regenerate Column

Wash and regenerate columns with buffer(s) and preserving agent(s)

Clean System

Choose from up to 9 buffers to clean sample and column lines

Condition Column
Load Sample

Deliver up to 1L of samples such as cell lysate or other biological fluids through columns

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